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周期素E2重組兔單克隆抗體
  • 產(chǎn)品貨號(hào)：
    BN42073R
  • 中文名稱：
    周期素E2重組兔單克隆抗體
  • 英文名稱：
    Rabbit anti-Cyclin E2 Monoclonal antibody
  • 品牌：
    Biorigin

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  • BN42073R-50ul

    50ul

    ￥2020.00

    交叉反應(yīng)：Human(predicted:Mouse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

  • BN42073R-100ul

    100ul

    ￥3240.00

    交叉反應(yīng)：Human(predicted:Mouse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

產(chǎn)品描述

英文名稱Cyclin E2
中文名稱周期素E2重組兔單克隆抗體
別    名CCN E2; CCNE 2; CCNE2; CCNE2 protein; CYC E2; CYCE 2; CYCE2; CyclinE2; G1/S-specific cyclin E2; G1/S-specific cyclin E2; CCNE2_HUMAN.  
研究領(lǐng)域腫瘤  細(xì)胞生物  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞周期蛋白  
抗體來源Rabbit
克隆類型Monoclonal
克 隆 號(hào)2B1
交叉反應(yīng)Human,  (predicted: Mouse, )
產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 Flow-Cyt=2ug/Test ICC=1:50 IF=1:50-200 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量44kDa
細(xì)胞定位細(xì)胞核 
性    狀Liquid
濃    度1mg/ml
免 疫 原Recombinant human Cyclin E2 protein, around C-terminal 100aa: 
亞    型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells.

Subunit:
"Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.

Subcellular Location:
Nucleus.

Tissue Specificity:
According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

Post-translational modifications:
Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway.

Similarity:
Belongs to the cyclin family. Cyclin E subfamily.

SWISS:
O96020

Gene ID:
9134

Database links:

Entrez Gene: 9134 Human

Entrez Gene: 12448 Mouse

Entrez Gene: 362485 Rat

Omim: 603775 Human

SwissProt: O96020 Human

SwissProt: Q7TMS8 Mouse

SwissProt: Q9Z238 Mouse

Unigene: 567387 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.